2024
Liu, Z. S.#, Sinha, S.#, Bannister, M., Song, A., Arriaga-Gomez, E., McKeeken, A. J., Bonner, E. A., Hanson, B. K., Sarchi, M., Takashima, K., Zong, D., Corral, V. M., Nguyen, E., Yoo, J., Chiraphapphaiboon, W., Leibson, C., McMahon, M. C., Rai, S., Swisher, E. M., Sachs, Z., Chatla, S., Stirewalt, D. L., Deeg, H. J., Skorski, T., Papapetrou, E. P., Walter, M. J., Graubert, T. A., Doulatov, S., Lee, S. C.*, Nguyen, D. H.* R-Loop Accumulation in Spliceosome Mutant Leukemias Confers Sensitivity to PARP1 Inhibition by Triggering Transcription-Replication Conflicts. Cancer Research, 84(4):577-597. doi: 10.1158/0008-5472.CAN-23-3239
#Co-authors, *co-correspondence
Somatic mutations in genes encoding RNA splicing factors are prevalent across various hematologic malignancies, including myelodysplastic syndromes and acute myeloid leukemia. Perturbed RNA splicing is often associated with an abnormal accumulation of RNA:DNA hybrid structures known as R-loops, depicted in the bottom left of the cover image. Spliceosome-mutant leukemias are dependent on PARP1 for survival due to its role in suppressing transcription–replication conflicts at R-loops, highlighting the potential therapeutic utility of PARP inhibitors in the treatment of hematologic malignancies.
2023
Laspata, N., Kaur, P., Mersaoui, S. Y., Muoio, D., Liu, Z. S., Bannister, M. H., Nguyen, H. D., Curry, C., Pascal, J. M., Poirier, G. G., Wang, H., Masson, J.-Y., Fouquerel, E. (2023). PARP1 associates with R-loops to promote their resolution and genome stability. Nucleic Acids Research, 51(5), 2215–2237. doi: 10.1093/nar/gkad066
White, III, R. E., Bannister, M., Day, A., Bergom, H. E., Tan, V. M., Hwang, J., Nguyen, H. D., Drake, J. M. Saracatinib synergizes with enzalutamide to downregulate AR activity in CRPC. Frontiers in Oncology, 13, 1210487. doi: 10.3389/fonc.2023.1210487
2022
Dator RP, Murray KJ, Luedtke MW, Jacobs FC, Kassie F, Nguyen HD, Villalta PW, and Balbo S. Identification of Formaldehyde-Induced DNA–RNA Cross-Links in the A/J Mouse Lung Tumorigenesis Model. Chemical Research in Toxicology 35(11)2025-36
2020
Matis DA, Zhang JM, Ouyang J, Nguyen HD, Genois MM, and Zou L. ATR Protects the Genome against R loops through a MUS81-Triggered Feedback Loop. Molecular Cell Oct 28. pii: S1097-2765(19)30770-1
2019
Nguyen HD, Zou L, and Graubert TA. Targeting R-loop-associated ATR response in myelodysplastic syndrome. Oncotarget 10(27)2581-82
2018
Nguyen HD*, Leong WY*, Li W, Reddy PNG, Sullivan JD, Walter MJ, Zou L, and Graubert TA. Spliceosome Mutations Induce R Loop-Associated Sensitivity to ATR Inhibitor in Myelodysplastic Syndrome. Cancer Research, 78(18):5363-74. (*co-first author).
Becker JR, Gallo D, Leung W, Croissant T, Thu YM, Nguyen HD, Starr TK, Brown GW, Bielinsky AK. Flap endonuclease overexpression drives genome instability and DNA damage hypersensitivity in a PCNA-dependent manner. Nucleic Acids Research. 46(11):5634-5650.
Kabeche L, Nguyen HD, Buisson R, Zou L. A Mitosis-specific and R-loop driven ATR pathway promotes faithful chromosome segregation. Science. 359(6371):108-114.
Nguyen HD and Zou L. Getting a Genomic View of DNA Replication Stress. Molecular Cell. Oct 18;72(2):201-203.
2017
Nguyen HD*, Yadav T*, Giri S, Saez B, Graubert TA, and Zou L. Functions of Replication Protein A as a Sensor of R Loop and a Regulator of RNaseH1. Molecular Cell.65(5):832-847. (*co-first author).
Yazinski SA, Comaills V, Buisson R, Genois MM, Nguyen HD, Ho CK, Todorova Kwan T, Morris R, Lauffer S, Nussenzweig A, Ramaswamy S, Benes CH, Haber DA, Maheswaran S, Birrer MJ, Zou L. ATR Inhibitors Overcome the Resistance of BRCA1-Deficient Cancer Cells to PARP Inhibition. Genes & Development. 31(3):318-332.
2016
Thu YM, Van Riper SK, Higgins L, Zhang T, Becker JR, Markowski TW, Nguyen HD, Griffin TJ, Bielinsky AK. Slx5/Slx8 Promotes Replication Stress Tolerance by Facilitating Mitotic Progression. Cell Reports 15(6):1254-65.
2015
Becker JR, Pons C, Nguyen HD, Costanzo M, Boone C, Myers CL, Bielinsky AK. Genetic Interactions Implicating Postreplicative Repair in Okazaki Fragment Processing. PLoS Genetics. 11(11):e1005659
Ouyang J, Garner E, Hallet A, Nguyen HD, Richman KA, Gill G, Smogorzewska A, and Zou L. Non-covalent interactions with SUMO and Ubiquitin orchestrate distinct functions of the SLX4 complex in DNA repair. Molecular Cell. 57(1):108-22.
2014
Wu CS, Ouyang J, Mori E, Nguyen HD, Maréchal A, Hallet A, Chen DJ, and Zou L. SUMOylation of ATRIP potentiate DNA damage signaling by boosting multiple protein interactions in the ATR pathway. Genes & Development. 28(13):1472-84.
Becker JR, Nguyen HD, Wang X, and Bielinsky AK. Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair. Cell Cycle. 13(11):1737-48.
Maréchal A, Li JM, Ji XY, Wu CS, Yazinski SA, Nguyen HD, Liu S, Jiménez AE, Jin J, Zou L. PRP19 Transforms into a Sensor of RPA-ssDNA After DNA Damage and Drives ATR Activation Via a Ubiquitin-Mediated Circuitry. Molecular Cell. 53(2):235-46.
2013
Nguyen HD, Becker J, Thu, YM, Costanzo M, Koch EN, Smith S, Myung KJ, Myers CL, Boone C, and Bielinsky AK. Unligated Okazaki Fragments Induce PCNA ubiquitination and a Requirement for Rad59-Dependent Replication Fork Progression. PLoS ONE.8(6):e66379.
Shiotani B, Nguyen HD, Håkansson P, Maréchal A, Ho CK, Tse A, Tahara H, and Zou L. Two Distinct Modes of ATR Activation Orchestrated by Rad17 and Nbs1. Cell Reports.3(5):1651-62.
2010
Das-Bradoo S*, Nguyen HD*, and Bielinsky AK. Damage-specific modification of PCNA. Cell Cycle.9(18):3674-9 (*co-first author).
Nguyen, HD and Bielinsky, AK. HMD2 ERKs PCNA. Journal of Cell Biology. (190):487-489.
Das-Bradoo S*, Nguyen HD*, Wood JL*, Ricke RM, Haworth JC, and Bielinsky AK. Defects in DNA Ligase I Trigger PCNA Ubiquitylation at Lys 107. Nature Cell Biology. 12(1):74-9 (*co-first author).
